The prospective national program MICCHADO (Molecular and Immunological Characterization of high risk CHildhood cancer at diagnOsis, during treatment and follow up; NCT03496402) aims for detailed molecular biological analyses including WES and RNAseq of high risk pediatric cancers at diagnosis. Plasma for sequential study of circulating tumor DNA for analysis of study of clonal evolution is performed for all patients. Furthermore, immunological characterization is is an important part of this program, with analysis of the tumor immune infiltrate and the immune profile of the host. To date MICCHADO has enrolled 580 of 600 planned patients, with end of recruitment planned in 03/2024. Designed as a research study, a workflow has been established to enable reports of the analyses in a clinical setting.

MICCHADO is based on a close collaboration between Institut Curie (sponsor; PI: Gudrun Schleiermacher) , Gustave Roussy (co-PI Claudia Pasqualini) and Centre Leon Berard (coPI Nadège Corradini) with active participation of all 30 centers of the SFCE.